Eppendorf ReferenceOperating Manual ®

95.1 Reference fix5.2 Reference variableModel /VolumeColorcodeepT.I.P.S. SystematicerrorRandomerrorμL μL%μL%μL12510102025501002002505001,0001,5002,000

10The technical data is only valid when the quoted eppendorf pipette tips are used.Tests carried out in accordance with ISO 8655 for piston-stroke pip

11The pipette can be individually labelled. The autoclavable blank adhesive label provided can be marked with a permanent marker and fits onto the ide

127.4 Dispensing liquid– Hold the tip at an angle against the inside wall of the tube.– Press down the control button slowly to the first stop (measur

13The serial number of the pipette is located on its control button.8.1 TestingDistilled water is dispensed from a pre-wetted tip into a tube and is t

14If the place where the pipette is used is at extremely high altitude, an adjustment must be made in line with the ambient air pressure. At 1,000 m a

15e Adjust control button by the volume determined.One revolution of the control button corresponds to the following values for water:Clockwise rotati

16d Pierce the calibration seal attached to the openingfor adjustments with Side B of the key provided.This destroys the seal which should be removed.

179.1 CareDepending on the frequency of use, all parts of the pipette should be cleaned from time to time in a soap solution or should be carefully wi

189.3 Decontamination prior to dispatchIf the Reference pipette is to be checked, repaired or calibrated by Eppendorf AG or another service partner, i

1Reference variabel / Reference adjustable0.1 – 2.5 μL0.5 – 10 μL2–20μL10–100 μL 20–200 μL 100 – 1000 μL 500 – 2500 μLReference fix1–50μL 100 μL 200 –

19Error Cause SolutionDroplets onthe inner wallof the pipette tip.– Uneven wetting of the plastic wall.– A pipette tip with poor wetting properties ha

20Control button jams, moves erratically.– Piston contaminated.– Seals contaminated.– Penetration of solvent vapors.– Clean piston and lubricate light

2110.1 If there is doubt that dispensing data are correctTo avoid dispensing errors, the precision and accuracy of the Reference pipettes need to be c

94MaintenanceI Exchanging the piston ...95II Exchanging the seals ...

95Eppendorf Reference® – Part B – Maintenance– Unscrew pipette at the central junction.– Press control button and hold down. Hold piston at the top of

96Removing the sealsFig. 2 – 6 on the following pages show you how to remove the seals. The numbers shown are identical with the numbers in the Orderi

9710 – 100 μL ( Fig. 3)Push the control button all the way down and pull off the ejector sleeve (13).Piston seal (4)– Unscrew pipette.– Discard seal (

9850 – 200 μL ( Fig. 4)Push the control button all the way down and pull off the ejector sleeve (13).Piston seal (4)– Unscrew pipette.– Remove piston

9950 – 250 μL ( Fig. 5)Push the control button all the way down and pull off the ejector sleeve (13).Piston seal (4)– Unscrew pipette at the central j

100100 – 1000 and 500 – 2500 μL (Fig. 6)Push the control button all the way down and pull off the ejector sleeve (13).Piston seal (4)– Unscrew pipette

2 Operating Manual ...4 Bedienungsanleitung ...

101Inserting the sealsPull new seal off the pin and push screw, spring, seal (with plastic part first) in that order onto side B of the wrench as show

102Ordering Information(Please see page 1 (Fig. 1) of this manual).Only parts with order numbers are available separately.Pipettes / spare partsModels

103Order numberInternationalOrder numberNorth America1Piston1 – 10 μL (gray control button) 4910 810.001 02247508820 μL 4910 811.00810 – 20 μL (yellow

104Order numberInternationalOrder numberNorth America5 Lower housing not sold separately6 Ejector, includes ejector spring (7)1 – 20 μL, incl. ejector

105Order numberInternationalOrder numberNorth America13 Ejector sleeve1–100 μL4910 845.000 022475827150 – 200 μL 022475843200 – 1000 μL 4910 846.00625

106(Please see page 1 (Fig. 1) of this manual).Only parts with order numbers are available separately.Pipettes / spare partsModels Order numberInterna

107Order numberInternationalOrder numberNorth America4 Piston seal10–100 μL, incl. screw (8), spring (9) 4910 820.007 02247528250–200 μL, with 2 O-r

108Order numberInternationalOrder numberNorth America12 Filling tube, (5 pieces, 1 wire punch)10–100 μL 4910 837.007 02247574650–200 μL, incl. 2 piece

109Order numberInternationalOrder numberNorth AmericaPipette carousel, incl. 6 pipette supports 3115 000.003 022444905Pipette holder (replacement for

110epT.I.P.S.(The packaging units stated represent the minimum ordering quantity).Length Color code Order numberInternationalOrder numberNorth America

3

111Length Color code Order numberInternationalOrder numberNorth AmericaBox, 1 box plus 96 tips0.1 – 10 μL 34 mm dark gray 0030 073.002 0224913000.1 –

112Length Color codeOrder numberInternationalOrder numberNorth AmericaSingles (Eppendorf Biopur), colorless, pyrogen-free, DNA-free, RNase-free, ATP-f

113Length ColorcodeOrder numberInternationalOrder numberNorth AmericaReloads, LoRetention PCR clean, 10 x 96 = 960 tips0.1 – 10 μL 34 mm dark gray 003

AUSTRALIA & NEW ZEALANDEppendorf South Pacific Pty. Ltd.Phone: +61 2 9889 5000Fax: +61 2 9889 5111E-mail: [email protected]: www.eppen

Your local distributor: www.eppendorf.com/worldwideEppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 538 01-0 · Fax: +49 40 538 01-556 · E-Mail: ep

4Eppendorf Reference® – Part A – Table of contentsOperating Manual1 Main Illustration ...

51Control button The control button is for setting the volume, dispens-ing and ejecting the pipette tip.2Volume display Displays the preset volume. 3L

62.1 Hazard icons2.2 Danger levelsThe danger level is part of a safety note and defines the potential consequences if the information contained therei

7WARNINGWARNING! Damage to health due to handling infectious liquids and pathogenic germs. Observe the national regulations for handling these substa

8NOTICENOTICE! Damage to device from missing pipette tips. Only use the Reference with attached pipette tips. NOTICENOTICE! Carry-over, contamination