Eppendorf FemtoJet Uživatelský manuál

FemtoJet ® Operating Manual eppendorf ® is a registered trademarkPrinted in Germany Your local distributor: www.eppendorf.com/worldwide Eppendorf AG

8 2 Device description 2.2 Short description Display Display of all relevant parameters and information. Function keysClean To apply the maximum pr

9 2 Device description Variable regulator In the normal operating mode, the parameters for injection are set by the three variable regulators. The a

10 2 Device description 2.3 Startup Diagram of rear of device– Electrical connection is effected via the mains socket with integrated mains switch

11 2 Device description The drainage valve of the pressure cylinder is switched several times at the beginning of the initialization phase. The pres

12 3 Mode of operation 3.1 Display, variable regulators and keypad Before operating the device for the first time, users should read this section c

13 3 Mode of operation Slight deviations between the nominal value (specified value) and displayed compensation pressure p c (actual value) may occ

14 3 Mode of operation 3.1.3 Keypad Manual injection is performed for as long as the key is held down. Injection pressure pi is used. In the seco

15 3 Mode of operation Auto / Manu To switch between manual and automatic, time-controlled injection. In the case of manual injection, timeis coun

16 3 Mode of operation Standbyin the case of a brief interruption When key is pressed briefly: The device switches to the Standby mode without d

17 3 Mode of operation 3.2 Hand control / foot control The functions of the hand/foot control correspond to the functions of the and keys on the

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18 3 Mode of operation 3.3 Connecting the capillary holder Universal capillary holder The universal capillary holder is used to locate the injectio

19 3 Mode of operation Other accessories Grip head 0, incl. 2 sets of O-rings and sealing washer.Using grip head 0 supplied, capillaries with an out

20 3 Mode of operation The usual values for the permanent compensation pressure P c are bet-ween 30 – 50 hPa (0,44 – 0,73 PSI) for Femtotips and si

21 3 Mode of operation 3.4.1 Compensation pressure p c The compensation pressure p c ensures that no medium flows into the capillary. Capillary

22 3 Mode of operation 3.4.3 Injection time t i Injection time t i gives the period for which injection pressure p i is maintained. t i is usua

233 Mode of operation3.4.5 Manual or automatic injectionIn the Manu operating mode, injection is performed for as long as the key or hand / foot co

243 Mode of operation3.5 The first injectionThe following are available: ● Adherent cells in a Petri dish (possibly on CELLocates to observe specific

253 Mode of operation3.6 Connecting an Eppendorf MicromanipulatorConnection to an Eppendorf Micromanipulator designed for automated injection is eff

263 Mode of operationFemtoJet (rear) with all connection options, with power unit 5171/InjectMan 51791 Foot control (optional) with Inject function2

273 Mode of operation3.7.1 Changing the capillaries3.7.2 Setting the pressure unit 3.7.3 Switching on/off the loudspeaker3.7.4 Switching on/off t

1 Safety precautions and application . . . . . . . . . . . . . . . . . . . 3 2 Device description . . . . . . . . . . . . . . . . . . . . . . .

283 Mode of operation3.7.5 Constant pressure3.7.6 Dewatering the pressure accumulatorFUNC 4This function can be used to apply a constant working pr

29 4.1 Cleaning Before cleaning the FemtoJet  , disconnect the plug.Ensure that no fluids enter into the inside of the injector in order to avoid sh

30 5 Troubleshooting 5.1 Potential errors – Pressure loss due to capillary not fitted with capillary holder connected.Solution: Connect the capillar

31 5 Troubleshooting key: Complete self-test– Checks compressor run time up to the point when a specific pressureis reached and thus for leaks in

32 5 Troubleshooting 5.2 Warnings As a rule, warnings are emitted if certain operating conditions are not (yet) reached or if an action is not poss

33 5 Troubleshooting 5.3 Error messages Error messages are emitted if certain operating conditions are not reached or the device records a deviatio

34 5 Troubleshooting Error 10 Supply pressure cannot be reachedThe compressor runs without the maximum supply pressure being reached.Possible cause:

35 5 Troubleshooting Error 13 Supply pressure does not rise Possible cause: Technical defect Solution: Service Error 14 Massive leak in pressure acc

36 5 Troubleshooting Error 21 Injection pressure has not been reached Possible cause: Capillary holder not attached or poorly attached. (Broken) cap

37 5 Troubleshooting Error 25 No drop in working pressure Possible cause: Technical defect Solution: Service Error 26 Working pressure leak rate is

2 5 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 305.1 Potential errors . . . . . . . . . . . . . . . . .

38 5 Troubleshooting Error 28 Working pressure leak rate is too highPossible cause: Capillary holder not attached or poorly attached. (Broken) capil

39 5 Troubleshooting Error 34 Valve 1 offset is too low Possible cause: Technical defect Solution: Service Error 35 Valve 1 offset is too high Possi

40Voltage/Frequency: 100 V – 240 V ± 10 %, 50 – 60 HzSet voltage: adapts automaticallyFuse for 100 – 240 V: T1.0 A, 250 V (2 pcs.)Power consumption:

417 Ordering informationOrder no. 5247 000.013 FemtoJet100 V – 240 V ± 10 %, 50 – 60 HzFully programmable microinjector with integrated pressure sup

427 Ordering informationOrder no. 5176 190.002 Universal capillary holder(incl. grip head 0, adapter for Femtotipsand spare O-rings)4910 000.018 Epp

437 Ordering informationOrder no.TransferMan NK 2Proportional micromanipulator for microinjection in suspension cells5188 000.012 Version Europe5188

447a Ordering information for North AmericaOrder no. FemtoJet100 V – 240 V ± 10 %, 50 – 60 HzFully programmable microinjector with integrated pressu

457a Ordering information for North AmericaOrder no. 920007392 Universal capillary holder(incl. grip head 0, adapter for Femtotipsand spare O-rings)

467a Ordering information for North AmericaOrder no.TransferMan NK 2Proportional micromanipulator for microinjection in suspension cells920000011 Ve

47 A Adapter for Femtotips  18Application 3, 4 C Capillary change 27Clean 8pressure 20function 22Cleaning 29Commands 46Compressor 21, 23Compensatio

3 ● Observe operating instructions! ● The device is only designed for use in the research lab! ● The FemtoJet is a lab device. It may only be operate

48 8 Index P Pressure– dewatering pressure accumulator 28– set pressure unit 26 Q Query 45 R Reset 9, 17function 16 S Safety precautions 3, 4Self

49Appendix AOperation via a computerCertain commands and queries (Section 6.2) can be transmitted to the unit using the optional cable/PC interface. A

50Appendix AIf the command is faulty, an error message is returned instead of the answer:"ERR=eee"ERR: Error=: Separationeee: Error numberLo

51Appendix ACommand to enter parameters[C]Code[xxx]Input parameter [ssssssssss]Meaning of input parameterSet compensationpressure pc 043 0; 5 – 6,000

52Appendix AError no.for incorrect operating constructionERR = [eee]Cause Solution 060 The first character is nota c or C.Check entry parameters. Chang

53Appendix AOther error numbers as well as the error cause and solution are described in Section 5, "Troubleshooting". During remote control

54Appendix AA_Bedien_Computer_en.fm Seite 54 Mittwoch, 27. September 2006 5:26 17

A_Bedien_Computer_en.fm Seite 55 Mittwoch, 4. Juli 2007 2:07 14

4 ● Use the unit only for the described purpose! The unit and in particular the high pressure of the Clean function may not be used for purposes othe

5 2 Device description The FemtoJet  enables minute quantities of liquid to be injected into cells. The relevant parameters (injection pressure p

6 2 Device description 2.1 Overview 1 Display – Displays relevant parameters during injection or settingof parameters.– Guides operator through fun

7 2 Device description Standard accessories:1 Injection tube, 2 m1 Universal capillary holder with grip head for capillary diameters of 1.0 mm